Complete nucleotide sequence of peanut clump virus RNA 1 and relationships with other fungus-transmitted rod-shaped viruses

Herzog, E. and Guilley, H. and Manohar, S.K. and et al, . (1994) Complete nucleotide sequence of peanut clump virus RNA 1 and relationships with other fungus-transmitted rod-shaped viruses. Journal of General Virology , 75 (11). pp. 3147-3155.

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The complete nucleotide sequence of RNA 1 of peanut clump furovirus (PCV) was determined by characterization of cloned cDNA and by direct RNA sequencing. The sequence is 5897 nucleotides in length and contained 3 long open reading frames (ORFs). The 5′-terminal proximal ORF has the potential to encode a polypeptide of Mr 130 942 (P131) containing methyltransferase and RNA helicase homologous domains and displaying homology with large nonstructural proteins of alpha-like viruses, which are known or thought to be involved in virus replication. The P131 ORF was followed in-frame by a 2nd ORF which was probably expressed by partial readthrough of the UGA termination codon of the P131 ORF to produce a polypeptide of Mr 191 044 (P191). The readthrough region of P191 contained the characteristic 'core' RNA polymerase motif, indicating that the PCV replicase proteins were expressed as a pair of overlapping proteins as in the tobamoviruses, tobraviruses and soil-borne wheat mosaic furovirus (SBWMV). Sequence comparisons indicated that P131 and P191 were most closely related to the replicase proteins of SBWMV and barley stripe mosaic hordeivirus (BSMV) but were only distantly related to the replicase of the beet necrotic yellow vein furovirus (BNYVV). The 3′-terminal proximal ORF encoded a putative polypeptide of Mr 14 556 (P15) which displayed homology to small cysteine-rich proteins of hordeiviruses and SBWMV. Four errors in the sequence of PCV RNA 2, previously published, are corrected. One of these changes caused 2 small ORFs near the 3′ terminus of RNA 2 to be fused together to create an ORF for a putative polypeptide of Mr 16 833 (P17) which displayed extensive homology with the 3rd protein of the triple gene block of BSMV RNA β.

Item Type: Article
Additional Information: Dr S N Nigam research collection - Box No:9
Uncontrolled Keywords: Nucleotide sequence, Peanut Clump virus RNA 1, Rod shaped virus
Author Affiliation: Institut de Biologie Moléculaire des Plantes du CNRS et de l'Université Louis Pasteur, France.
Subjects: Plant Protection
Crop Improvement
Divisions: Groundnut
Depositing User: Mr Arbind Seth
Date Deposited: 28 Mar 2013 14:39
Last Modified: 28 Mar 2013 14:39
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