Long-term regeneration in callus culture of paisa (Echinochloa frumentacea Link.)

Bobkov, S.V. (2005) Long-term regeneration in callus culture of paisa (Echinochloa frumentacea Link.). International Sorghum and Millets Newsletter, 46. pp. 120-122.

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A study was conducted to evaluate the long term regeneration of paisa (Echinochloa frumentacea) through callus culture. Mature seeds of the paisa cultivar Udalaya were sterilized in 70% ethanol and 0.5-2.0% chlorhexidine sodium digluconate and were placed on 2KC media which contained salts according to the MS protocol, vitamins according to the B5 media, and 100 mg/litre myo-inositol, 4 g/litre sucrose, 2 mg/litre glycine, 6 g/litre agar and 2 mg/litre 2,4-D. Various levels of 2,4-D was also used in the 2KC media. The MSB media was similar to 2KC without 2,4-D. Seed sprouted in the 2KC media while on the other seed, formation of calluses was observed. Segments of calluses with white, compact tissue and with regenerated shoots were transferred onto fresh 2KC media. As a result of their transfer and development on fresh media, callus tissues of 4 types were formed: type A (calluses with non-differentiated tissue on the border with the media, white compact tissues on the surface and presence of the process of regeneration); type B (calluses with non-differentiated tissue and presence of the regeneration process); type C (calluses with predominance of the regeneration process and lack of proliferation of callus mass); and type D (calluses with predominance of proliferation of a non-differentiated tissue). Transfer of type C and D callus from the 2KC media onto an MSB media resulted in a decrease of callus mass proliferation and an increase of shoots without roots. Plants without root systems, obtained on MSB did not tolerate transfer to unsterile conditions. Periodic transfer of the organized segments of type A and B calluses onto fresh 2KC has maintained the calluses and regeneration potential to regenerate plants over a long period of time. Regenerated plants were transferred into soil, grown in the greenhouse, flowered and produced seeds. The results indicate that tissue culture could be optimized to maintain viable callus for many years of paisa, which can lead to the regeneration of clones for use in breeding programmes and can generate somaclonal variation for use in those programmes.

Item Type: Article
Author Affiliation: All-Russia Research Institute of Legumes and Groat Crops, p/b Streletskoye, Orel
Subjects: Crop Improvement > Biotechnology
Divisions: Millet
Depositing User: Sandhya Gir
Date Deposited: 14 Dec 2010 17:37
Last Modified: 29 Dec 2010 19:47
URI: http://eprints.icrisat.ac.in/id/eprint/1006

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