Identification of SSR Markers Associated with N2-Fixation Components in Soybean [Glycine max (L.) Merr.]

Tanya, P. and Srinives, P. and Toojinda, T. and et al, . (2005) Identification of SSR Markers Associated with N2-Fixation Components in Soybean [Glycine max (L.) Merr.]. Korean Journal of Genetics, 27 (4). pp. 351-359.

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Regulation of nodulation and nitrogen fixation in legume plants relies mainly on the interaction between the host plant and the symbiotic bacteria. The purpose of this study was to identify quantitative trait loci (QTLs) for nodulation and nitrogen fixation characters in a soybean [Glycine max (L.) Merr.] population. Simple sequence repeat (SSR) markers were utilized to identify QTLs for the number of nodules per plant, nodule fresh and dry weight per plant, plant dry weight, and acetylene reduction activity (ARA) in 136 F2-derived recombinant inbred lines (RILs) from the cross ‘SJ2’ x ‘Suwon 157’. A genetic linkage map was constructed using 85 simple sequence repeat (SSR) markers onto 20 linkage groups covering 1094 cM. Using single-factor analysis of variance (SF-ANOVA) and multiple regression analysis, five QTLs were associated with the number of nodules per plant. For the QTLs linked to linkage group (LG) O, Satt529 on LG J, Satt440 on LG I, and Satt157 on LG D1b+W the positive alleles were derived from ‘Suwon 157’, whereas at the QTL linked to Satt385 on LG A1 the positive allele was from ‘SJ2’. The QTLs conditioning the nodule fresh weight were linked to Sat_108 and Sat_274 in LG O, and Sct_001 in LG J, with the positive alleles derived from ‘Suwon 157’, whereas the positive allele of Satt545 on LG A1 was derived from ‘SJ2’. Sat_274 and Sat_108 on LG O, and Satt260 on LG K were associated with QTL conditioning nodule dry weight, with the positive allele of ‘Suwon 157’. There were four QTLs associated with plant dry weight, viz. Sat_274 and Sat_038 on LG O, Sct_001 on LG J, and Satt440 on LG I, with all the positive alleles from ‘Suwon 157’. Finally, Sat_274 on LG O and Satt157 on LG D1b+W were linked to QTLs conditioning ARA, with the positive alleles were from ‘Suwon 157’. These data demonstrated that effective marker-assisted selection may be feasible for improving nodulation and nitrogen fixation in soybean. The existence of a major QTL on LG O conditioning greater than 10% of the phenotypic variation in nodulation and nitrogen fixation characters provides an opportunity to select progeny lines in a segregating population.

Item Type: Article
Uncontrolled Keywords: Soybean, Quantitative trait loci, SSR marker, N2-fixation.
Author Affiliation: Department of Agronomy, Faculty of Agriculture, Kasetsart University, Thailand DNA Technology Laboratory, National Center for Genetic Engineering and Biotechnology, Kasetsart University, Thailand Department of Plant Science, College of Agriculture and Life Science, Seoul National University, Korea
Subjects: Plant Protection
Crop Improvement
Divisions: Soyabean
Depositing User: Mr Arbind Seth
Date Deposited: 03 Apr 2013 09:37
Last Modified: 03 Apr 2013 09:37

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