Construction of Molecular Linkage Maps Using SRAP Markers in Sweetpotato

Ai-Xian, LI and Qing-Chang, LIU and Qing-Mei, WANG and et al, . (2010) Construction of Molecular Linkage Maps Using SRAP Markers in Sweetpotato. Acta Agronomica Sinica, 36 (8). pp. 1286-1295.

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The objective of this study was to construct a linkage map of sweetpotato [Ipomoea batatas (L.) Lam.] based on Chinese cultivars with higher marker density than previous maps. Cultivars Luoxushu 8 (female parent) and Zhengshu 20 (male parent) with obvious differences in starch content, yield, β-carotene content, and disease resistance were crossed to produce a segregation population with 240 F1 individuals. Among the total 800 sequence-related amplified polymorphism (SRAP) markers, 770 and 523 markers showed polymorphism in the female and male parents, respectively. Using the “double pseudo testcross” strategy, 2 linkage maps were constructed by means of JoinMap 3.0. The linkage maps were composed of 473 markers in 81 linkage groups for Luoxushu 8 and 328 markers in 66 linkage groups for Zhengshu 20. The former map covered 5802.46 cM with an average marker interval of 10.16 cM, and latter one covered 3967.90 cM with an average of 12.02 cM. Both linkage maps may facilitate marker-assisted selection, gene localization, and gene cloning in sweetpotato.

Item Type: Article
Additional Information: This study was supported by the National High Technology Research and Development Program of China (2006AA100107), the Earmarked Fund for Modern Agro-industry Technology Research System (Sweetpotato), the National Key Technologies R&D Program of China (2006BAD01A06), the Program of Introducing International Super Agricultural Science and Technology (2006-G21-02), Public Agricultural Industry Program (nyhyzx07-012-03), and the Natural Science Foundation of Shandong Province, China (Q2006D10).
Uncontrolled Keywords: sweetpotato; molecular linkage map; SRAP marker
Author Affiliation: Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis and Utilization, Ministry of Education, China Agricultural University, Beijing 100193, China.
Subjects: Crop Improvement
Divisions: Other Crops
Depositing User: Mr Balakrishna Garadasu
Date Deposited: 03 Apr 2013 13:53
Last Modified: 03 Apr 2013 13:53
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