Genetic diversity analysis in sorghum germplasm as estimated by AFLP, SSR and morpho-agronomical markers

Geleta, N. and Labuschagne, M.T. and Viljoen, C.D. (2006) Genetic diversity analysis in sorghum germplasm as estimated by AFLP, SSR and morpho-agronomical markers. Biodiversity and Conservation, 15 (10). pp. 3251-3265.

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A comparison of the different methods of the estimation of genetic diversity is important to evaluate their utility as a tool in germplasm conservation and plant breeding. Amplified fragment length polymorphism (AFLP), microsatellites or SSR and morphological traits markers were used to evaluate 45 sorghum germplasm for genetic diversity assessment and discrimination power. The mean polymorphism information content (PIC) values were 0.65 (AFLPs) and 0.46 (SSRs). The average pairwise genetic distance estimates were 0.57 (morphological traits), 0.62 (AFLPs) and 0.60 (SSRs) markers data sets. The Shannon diversity index was higher for morphological traits (0.678) than AFLP (0.487) and SSR (0.539). The correlation coefficients obtained by the Mantel matrix correspondence test, which was used to compare the cophenetic matrices for the different markers, showed that estimated values of genetic relationship given for AFLP and SSR markers, as well as for morphological and SSR markers were significantly related (p <0.001). However, morphological and AFLP data showed non-significant correlation (p >0.05). Both data sets from AFLP and SSR allowed all accessions to be uniquely identified; two accessions could not be distinguished by the morphological data. In summary, AFLP and SSR markers proved to be efficient tools in assessing the genetic variability among sorghum genotypes. The patterns of variation appeared to be consistent for the three marker systems, and they can be used for designing breeding programmes, conservation of germplasm and management of sorghum genetic resources.

Item Type: Article
Additional Information: SNNigam Collection
Uncontrolled Keywords: AFLP, Genetic diversity, Germplasm, Morphology, Sorghum, SSR/microsatellites
Author Affiliation: Department of Plant Sciences, College of Agriculture, Alemaya University, Ethiopia.
Subjects: Crop Improvement
Divisions: Sorghum
Depositing User: Mr Arbind Seth
Date Deposited: 17 Apr 2013 08:57
Last Modified: 17 Apr 2013 08:57
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