High-Oleic Peanut Oils Produced by HpRNA-Mediated Gene Silencing of Oleate Desaturase

Yin, D. and Deng, S. and Zhan, K. and Cui , D. (2007) High-Oleic Peanut Oils Produced by HpRNA-Mediated Gene Silencing of Oleate Desaturase. Plant Molecular Biology Reporter , 25 (3-4). pp. 154-163.

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The quality of peanut oil largely depends on the quantity of oleic (18:1) and linoleic acids (18:2). These two acids comprise more than 80% of the total fatty acids in peanuts. The oleate desaturase (FAD2) gene is important for maintaining high oleic acid content. A partial conservative sequence of the FAD2 gene from peanut was selected. The sense and antisense 260-bp fragments were amplified and subcloned into pFGC1008 binary expression vectors. A total of 21 transgenic plants were obtained via Agrobacterium-mediated transformation. The resulting down-regulation of the FAD2 gene resulted in a 70% increase in oleic acid content in the seeds of transformed plants compared with a 37.93% increase in untransformed plants. The results demonstrated that the target genes were likely suppressed by hpRNA interference, a pathway capable of achieving phenotypic changes. The silencing of FAD2 enabled the development of peanut oils having novel combinations of oleic acid content that can be used in high-value applications, making this approach a reliable technique for the genetic modification of seed quality and the potential for enhancement of other traits as well

Item Type: Article
Additional Information: SNNigam Collection
Uncontrolled Keywords: Oleate desaturase . RNA interference . Edible oil . Transgenic plants
Author Affiliation: Department of Agronomy, Henan Agricultural University, 450002, Zhengzhou, China
Subjects: Crop Improvement
Divisions: Groundnut
Depositing User: Mr B Krishnamurthy
Date Deposited: 18 Apr 2013 05:02
Last Modified: 18 Apr 2013 05:02
Official URL: http://dx.doi.org/10.1007/s11105-007-0017-0
URI: http://eprints.icrisat.ac.in/id/eprint/10265

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