Rapid and sensitive assay for the phytotoxin rhizobitoxine

Ruan, X. and Peters, N.K. (1991) Rapid and sensitive assay for the phytotoxin rhizobitoxine. Applied and Environmental Microbiology, 57 (7). pp. 2097-2100.

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Rhizobitoxine is a phytotoxin synthesized by some strains of the legume symbiont genus Bradyrhizobium and the plant pathogen Pseudomonas andropogonis. We demonstrate here a new enzymatic assay which is 100-fold more sensitive than previous assays and can detect as little as 1.0 pmol of rhizobitoxine. The assay is based on the inhibition of Salmonella typhimurium beta-cystathionase by rhizobitoxine. Interestingly, beta-cystathionase from Bradyrhizobium japonicum is insensitive to rhizobitoxine at concentrations lower than 75 microM.

Item Type: Article
Author Affiliation: Department of Agronomy, Ohio State University, Columbus 43210-1002
Subjects: Plant Protection
Divisions: General
Depositing User: Mr B Krishnamurthy
Date Deposited: 15 May 2013 04:07
Last Modified: 15 May 2013 04:07
Official URL: http://aem.asm.org/content/57/7/2097.abstract
URI: http://eprints.icrisat.ac.in/id/eprint/10541

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