Chlorophyll Determination in Intact Tissues Using N,N-Dimethylformamide

Moran, R. and Porath, D. (1980) Chlorophyll Determination in Intact Tissues Using N,N-Dimethylformamide. Plant Physiology, 65 (3). pp. 478-479.

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Photosynthetic pigments from etiolated cucumber (Cucumis sativus var. Beit Alpha improved, Hazera Co., Gedera) cotyledons were extracted by direct immersion of the intact cotyledons into the solvent N,N-dimethylformamide (DMF). The solvent is especially efficient when pigment concentration is low; time and tools are saved and the loss of pigment that usually occurs in more complicated extraction procedures is prevented. The specific absorption coefficient of chlorophyll a in DMF was also determined

Item Type: Article
Author Affiliation: Department of Botany, The George S. Wise Faculty for Life Sciences, Tel Aviv University, Ramat Aviv, Israel
Subjects: Plant Physiology and Biochemistry > Plant Physiology
Divisions: Other Crops
Depositing User: Ms K Syamalamba
Date Deposited: 20 May 2013 05:31
Last Modified: 20 May 2013 05:31
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