Genetic diversity revealed utility of SSR markers in classifying parental lines and elite genotypes of sorghum (Sorghum bicolor L. Moench)

Ganapathy, K.N. and Gomashe, S.S. and Rakshit, S. and Prabhakar, B. and et al, . (2012) Genetic diversity revealed utility of SSR markers in classifying parental lines and elite genotypes of sorghum (Sorghum bicolor L. Moench). Australian Journal of Crop Sciences, 6 (11). pp. 1486-1493.

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Abstract

Genetic diversity among 82 rainy and post-rainy sorghum genotypes from India was studied using a set of 35 SSR markers distributed across all the linkage groups. A total of 198 alleles were recorded with an average of 5.71 per primer pair. The polymorphism information content (PIC) values ranged from 0.02 (Xisep 0310) to 0.86 (sb5-206) with a mean of 0.49, indicating high discriminating ability of the SSR markers used. Jaccard’s similarity coefficients and cluster analysis revealed substantial diversity among the genotypes. Very high estimate of fixation index (FST = 0.35, P= 0.001) was obtained when genotypes were structured as rainy and post-rainy season adaptation, and a much higher estimate (FST = 0.40, P= 0.001) was obtained when the genotypes were classified as varieties, maintainers, restorers and germplasm lines. This indicates strong distinction based on usage groups. Pairwise FST values based on usage groups corresponded well with the Nei’s genetic distances. We were able to distinguish parental lines of hybrids based on their fertility groups, especially lines from rainy season sorghum, using SSR markers. To the best of our knowledge, this is the first report in sorghum demonstrating the utility of SSR markers in classifying lines based on their fertility groups. Our results have significant implications in classifying parental lines into heterotic groups for their use in heterosis breeding. The divergent maintainer and restorer lines identified based on Jaccard’s similarity coefficients could serve as effective candidates for hybrid development.

Item Type: Article
Uncontrolled Keywords: genetic variability, heterotic groups, parental lines, rainy, post-rainy, sorghum
Author Affiliation: Directorate of Sorghum Research, Hyderabad 500 030, Andhra Pradesh, India
Subjects: Statistics and Experimentation
Crop Improvement
Divisions: Sorghum
Depositing User: Mr B Krishnamurthy
Date Deposited: 25 May 2013 14:23
Last Modified: 25 May 2013 14:23
Official URL: http://www.cropj.com/november2012.html
URI: http://eprints.icrisat.ac.in/id/eprint/10605

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