Comparison of PCR-RFLP analysis of the ITS region with morphological criteria of various strains of Dunaliella

González, M.A. and Gómez, P.I. and Montoya, R. (1998) Comparison of PCR-RFLP analysis of the ITS region with morphological criteria of various strains of Dunaliella. Journal of Applied Phycology, 10 (6). pp. 573-580.

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The genus Dunaliella comprises 28 species defined primarily by morphological and physiological criteria, which vary considerably depending on growth conditions. Concomitantly, the taxonomic status of various species is uncertain. To confirm the taxonomic identity and to better understand the relationship within Dunaliella, seven taxa ( D. salina, D. bardawil, D. tertiolecta, D. parva, D. viridis, D. lateralis, D. peircei) were compared using RFLP analysis of the nuclear rDNA repeats, specifically the internal transcribed spacer regions, including the 5.8S rRNA gene. Volvox aureus was used as an outgroup. A single ITS PCR amplification product was obtained for each taxon. An ITS fragment of ca. 640 bp was present in all the taxa within the subgenus Dunaliella, except for D. salina CCMP 1303 (ca. 540 bp) and D. lateralis (subgenus Pascheria) (ca. 600 bp). A cluster analysis based on the presence or absence of bands generated by digestion of the PCR product with 8 restriction endonucleases (DpnI, HhaI, EcoRI, PvuII, TaqI, HaeIII, MspI, StyI) revealed no correlation between the genetic relationship inferred from the ITS-RFLP data and the morpho-physiological attributes used for taxonomy. In addition, differences in morphology, physiology and in the length and restriction fragment patterns of the ITS region of D. salina CCMP 1303 suggest that this strain does not belong to Dunaliella.

Item Type: Article
Uncontrolled Keywords: Dunaliella, ITS, PCR, RFLP, strains, taxonomy
Author Affiliation: Departamento de Botánica, Facultad de Ciencias Naturales y Oceanográficas, Chile
Subjects: Crop Improvement
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 27 May 2013 08:17
Last Modified: 27 May 2013 08:17
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