Genomic inversion caused by gamma irradiation contributes to downregulation of a WBC11 homolog in bloomless sorghum

Mizuno, H. and Kawahigashi, H. and Ogata, J. and et al, . (2013) Genomic inversion caused by gamma irradiation contributes to downregulation of a WBC11 homolog in bloomless sorghum. Theoretical and Applied Genetics, 126 (6). pp. 1513-1520.

[img] PDF - Published Version
Restricted to ICRISAT researchers only


Epicuticular wax (bloom) plays important roles in protecting the tissues of sorghum (Sorghum bicolor (L.) Moench) plants from abiotic stresses. However, reducing wax content provides resistance to greenbug and sheath blight—a useful trait in agricultural crops. We generated a sorghum bloomless (bm) mutant by gamma irradiation. One bm population segregated for individuals with and without epicuticular wax at a frequency of 72:22, suggesting that the bm mutation was under the control of a single recessive nuclear gene. Genes differentially expressed in the wild-type and the bm mutant were identified by RNA-seq technology. Of the 31 downregulated genes, Sb06g023280 was the most differentially expressed and was similar to WBC11, which encodes an ABC transporter responsible for wax secretion in Arabidopsis. An inversion of about 1.4 Mb was present in the region upstream of the Sb06g023280 gene in the bm mutant; it is likely that this inversion changed the promoter sequence of the Sb06g023280 gene. Using genomic PCR, we confirmed that six independent F2 bm mutant-phenotype plants carried the same inversion. Therefore, we concluded that the inversion involving the Sb06g023280 gene inhibited wax secretion in the bloomless sorghum.

Item Type: Article
Additional Information: This work was supported by a Grant from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Genomics for Agricultural Innovation, SOR-0006).
Author Affiliation: Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences (NIAS), 1-2 Kannondai 2-chome, Tsukuba, Ibaraki, 305-8602, Japan
Subjects: Crop Improvement > Genetics/Genomics
Divisions: Sorghum
Depositing User: Mr Siva Shankar
Date Deposited: 29 May 2013 04:50
Last Modified: 29 May 2013 04:50
Official URL:

Actions (login required)

View Item View Item