Additive effects of three auxins and copper on sorghum in vitro root induction

Liu, G. and Gilding, E.K. and Godwin, I.D. (2013) Additive effects of three auxins and copper on sorghum in vitro root induction. In Vitro Cellular & Developmental Biology - Plant , 49 (2). pp. 191-197.

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A healthy root system is vital for tissue culture plantlet survival and rapid adaptation from the in vitro microenvironment to glasshouse conditions. Optimization of the root induction medium is an effective way to promote root induction and elongation. Levels of three auxins (α-naphthaleneacetic acid [NAA], 3-indoleacetic acid [IAA], and 3-indolebutyric acid [IBA]) and copper sulfate (CuSO4) have been investigated in a series of experiments with a sorghum inbred line, Tx430. Significant improvement in root proliferation and shoot growth were observed on Murashige and Skoog (MS) medium supplemented with 1 μmol/L CuSO4, 1 mg/L NAA, 1 mg/L IAA, and 1 mg/L IBA. On average, one explant (the original in vitro-derived shoot) of Tx430 regenerated 56.7 roots, which was 20-fold higher on the optimal medium than on MS control medium. Another tested genotype SA281 showed similar response patterns as Tx430 across media. In addition, 100% of Tx430 and SA281 plantlets originating from the optimized root induction medium all survived after being transferred to potting soil in the glasshouse. The results demonstrate that a combination of auxins (NAA, IAA, and IBA) and CuSO4 together at optimal concentrations provide additive effects on promoting root proliferation and explant growth of in vitro sorghum in root induction medium, and subsequently resulted in 100% survival rate of plantlets ex tissue culture. Compared with two published and frequently used root induction media, the optimized medium significantly enhanced root induction and plantlet growth

Item Type: Article
Uncontrolled Keywords: Sorghum bicolor . Auxin . Tissue culture . Copper sulfate . Root induction medium
Author Affiliation: School of Agriculture and Food Sciences, The University of Queensland, Brisbane, QLD, 4072, Australia
Subjects: Plant Protection
Soil Science and Microbiology
Divisions: Sorghum
Depositing User: Mr B Krishnamurthy
Date Deposited: 05 Jun 2013 10:19
Last Modified: 05 Jun 2013 10:19
Official URL:

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