Polymerase chain reaction with single-sided specificity: analysis of T cell receptor delta chain

Loh, E.Y. and Elliott, J.F. and Cwirla, S. and et al, . (1989) Polymerase chain reaction with single-sided specificity: analysis of T cell receptor delta chain. Science, 243 (4888). pp. 217-220.

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Abstract

In the polymerase chain reaction (PCR), two specific oligonucleotide primers are used to amplify the sequences between them. However, this technique is not suitable for amplifying genes that encode molecules where the 5' portion of the sequences of interest is not known, such as the T cell receptor (TCR) or immunoglobulins. Because of this limitation, a novel technique, anchored polymerase chain reaction (A-PCR), was devised that requires sequence specificity only on the 3' end of the target fragment. It was used to analyze TCR delta chain mRNA's from human peripheral blood gamma delta T cells. Most of these cells had a V delta gene segment not previously described (V delta 3), and the delta chain junctional sequences formed a discrete subpopulation compared with those previously reported.

Item Type: Article
Author Affiliation: Departments of Medicine and Microbiology and Immunology, Stanford University School of Medicine, CA 94305-5402.
Subjects: Crop Improvement
Plant Physiology and Biochemistry > Biochemistry
Social Sciences > Plant Physiology and Biochemistry > Biochemistry
Divisions: General
Depositing User: Mr Balakrishna Garadasu
Date Deposited: 25 Jun 2013 08:39
Last Modified: 25 Jun 2013 08:39
Official URL: http://dx.doi.org/10.1126/science.2463672
URI: http://eprints.icrisat.ac.in/id/eprint/10726

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