Ahmed, F.E. (2002) Detection of genetically modified organisms in foods. Trends in Biotechnology, 20 (5). pp. 215-223.
Legislation enacted worldwide to regulate the presence of genetically modified organisms (GMOs) in crops, foods and ingredients, necessitated the development of reliable and sensitive methods for GMO detection. In this article, protein- and DNA-based methods employing western blots, enzyme-linked immunosorbant assay, lateral flow strips, Southern blots,qualitative-, quantitative-, real-time- and limiting dilution-PCR methods,are discussed. Where information on modified gene sequences is not available, new approaches, such as near-infrared spectrometry, might tackle the problem of detection of non-approved genetically modified (GM) foods. The efficiency of screening, identification and confirmation strategies should be examined with respect to false-positive rates, disappearance of marker genes, increased use of specific regulator sequences and the increasing number of GM foods.
|Author Affiliation:||Dept of Radiation Oncology, Leo W. Jenkins Cancer Center, The Brody School of Medicine, LSB 014, East Carolina University, Greenville,NC 27858, USA|
|Subjects:||Crop Improvement > Genetics|
Crop Improvement > Plant Breeding
Crop Improvement > Biotechnology
|Depositing User:||Sandhya Gir|
|Date Deposited:||18 Oct 2010 07:43|
|Last Modified:||18 Oct 2010 07:45|
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