Lysis and biological control of Aspergillus niger by Bacillus subtilis AF 1

Podile, A.R. and Prakash, A.P. (1996) Lysis and biological control of Aspergillus niger by Bacillus subtilis AF 1. Canadian Journal of Microbiology , 42 (6). pp. 533-538.

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A biocontrol rhizobacterial strain of B. subtilis AF 1 grown for 6 h was coinoculated with A. niger at different time intervals. Microscopic observations revealed the adherence of bacterial cells to the fungal mycelium. Bacterial cells multiplied in situ and colonized the mycelial surface. Growth of AF 1 resulted in damage to the cell wall, followed by lysis. AF 1 inoculation into media containing A. niger at 0, 6 and 12 h suppressed >90% fungal growth, while in 18- and 24-h cultures fungal growth inhibition was 70 and 56%, respectively, in terms of dry weight. In dual culture, fungal growth was not accompanied by formation of spores. The mycelial preparation of A. niger, as principal carbon source, supported the growth of B. subtilis as much as chitin. Extracellular protein precipitate from B. subtilis culture filtrate had a significant growth-retarding effect on A. niger. Groundnut seeds bacterized with B. subtilis showed a reduced incidence of crown rot in A. niger infested soil, suggesting a possible role of B. subtilis in biological control of A. niger

Item Type: Article
Additional Information: SNNigams collection
Author Affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad - 500 046, India.
Subjects: Plant Protection
Divisions: Groundnut
Depositing User: Mr T L Gautham
Date Deposited: 08 Jul 2013 13:53
Last Modified: 08 Jul 2013 13:53
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