Random amplified polymorphic DNA analysis of Australian rice (Oryza sativa L.) varieties

Ko, H.L. and Cowan, D.C. and Henry, R.J. and Graham , G.C. and Blakeney, A.B. and Lewin, L.G. (1994) Random amplified polymorphic DNA analysis of Australian rice (Oryza sativa L.) varieties. Euphytica , 80 (3). pp. 179-189.

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The genetic relationships between rice varieties were analysed by using the polymerase chain reaction (PCR), with arbitrary oligonucleotide primers in the random amplified polymorphic DNA (RAPD) method. PCR with 22 arbitrary primers applied to 37 varieties produced 144 useful markers, of which 67% were polymorphic. Thus, with selected primers sufficient polymorphism could be detected to allow identification of individual varieties. Visual examination of electrophoresis gels and analysis of banding patterns confirmed that commercial Australian and USA lines and their relatives were very closely related, with similarity indices of 88–97%. Three varieties originating from more distant geographical centres were easily distinguished, producing variety-specific amplification profiles and expressing a lower similarity index of 80% to all other varieties tested. PCR offers a potentially simple, rapid and reliable method for rice genotype identification and recognition of lines that could contribute genetic diversity to new commercial varieties.

Item Type: Article
Additional Information: SNNigams collection
Author Affiliation: Queensland Department of Primary Industries, Queensland Agricultural Biotechnology Centre, Gehrmann Laboratories, University of Queensland, Qld 4072, Australia.
Subjects: Crop Improvement
Divisions: Other Crops
Depositing User: Mr T L Gautham
Date Deposited: 13 Jul 2013 13:28
Last Modified: 13 Jul 2013 13:28
Official URL: http://dx.doi.org/10.1007/BF00039649
URI: http://eprints.icrisat.ac.in/id/eprint/11020

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