Simple Method of Isolation and Purification of α-Galactosides from Legumes

Gulewicz, P. and Ciesiołka, D. and Frias, J. and et al, . (2000) Simple Method of Isolation and Purification of α-Galactosides from Legumes. Journal of Agricultural and Food Chemistry, 48 (8). pp. 3120-3123.

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A simple method for the isolation and purification of α-galactosides, raffinose family oligosaccharides (RFOs), from legumes has been developed. The method includes (i) imbibition of seeds, (ii) extraction with 50% ethanol, (iii) precipitation of RFOs, (iv) purification of RFOs on diatomaceous earth and charcoal, and (v) cation-exchange chromatography. The described method allows one to obtain high purity RFO preparations (90% for lentil and 80% for pea seeds, determined by HPLC-RI analysis) in the form of white, fine powder. Yields of α-galactosides isolated from 100 g of seeds of lentil and pea were 5.6 and 4.3 g, respectively.

Item Type: Article
Uncontrolled Keywords: α-Galactosides; raffinose family oligosaccharides (RFOs); lentil; pea
Author Affiliation: Department of Biotechnology and Food Microbiology, Agricultural University, Mazowiecka 48, 60-623 Poznan´ , Poland,
Subjects: Postharvest Management > Food Technology
Divisions: Other Crops
Depositing User: Mr Siva Shankar
Date Deposited: 12 Jul 2013 07:18
Last Modified: 12 Jul 2013 07:18
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