Isaac-CACTA transposons: new genetic markers in maize and sorghum

Lee, Ju Kyong and Kwon, Soon-Jae and Park, Kyong-Cheul and Kim, Nam-Soo (2005) Isaac-CACTA transposons: new genetic markers in maize and sorghum. Genome, 48 (3). pp. 455-460.

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CACTA is an En/Spm transposon superfamily present in high copy number in plant genomes, and Isaac is a subfamily of the CACTA superfamily. The TIR sequence of the Isaac subfamily was used for the purpose of transposon display (TD) in maize and sorghum. The Isaac TD produced 50–80 amplified fragments, depending on the primer combination, and the amplification profile was highly polymorphic among maize inbred lines. Isaac TD-based phylogenetic clustering distinguished the maize inbred lines according to their lineages and was consistent with the results of phylogenetic reports derived from other marker techniques by others researchers. The Isaac TD profile proved to be highly reproducible with different brands of Taq DNA polymerases and thermocyclers. The Isaac TD was also applied to recombinant inbred lines to assess genetic segregation; we observed 40–50 recordable segregation markers, depending on the primer combination. These Isaac TD markers segregated mostly as dominant markers, although, in a few cases, non-parental bands were observed in the segregating populations. In addition, the Isaac TD was very successful in the amplification of sorghum accessions. Therefore, the Isaac TD may provide another useful protocol for genetic analysis in maize and sorghum.

Item Type: Article
Author Affiliation: Plant Biotechnology Program, Division of Biotechnology, Kangwon National University, Chunchon, Korea, 200-701
Subjects: Crop Improvement > Genetics/Genomics
Divisions: Maize
Depositing User: Sandhya Gir
Date Deposited: 22 Dec 2010 21:11
Last Modified: 29 Dec 2010 19:34
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