Variation in di-nitrogen fixation among Andean bean (Phaseolus vulgaris L.) genotypes grown at low and high levels of phosphorus supply

Christiansen, I. and Graham, P. H. (2002) Variation in di-nitrogen fixation among Andean bean (Phaseolus vulgaris L.) genotypes grown at low and high levels of phosphorus supply. Field Crops Research, 73 (2-3). pp. 133-142.

[img] PDF - Published Version
Restricted to ICRISAT researchers only


An initial screening of 51 genotypes of Phaseolus vulgaris identified bean accessions with good growth and nodule development at low levels of soil phosphorus (P). Seeds of these accessions were inoculated with Rhizobium, and grown in a peat-based medium to which rock phosphate was added so as to supply 5 or 140 μM P in the soil solution. Harvests were made 17–45 days after planting and nodulation, N2 fixation, dry matter production, N and P concentration, and soluble carbohydrate and starch levels determined. Seed yields were also obtained. There was a strong correlation between nodule fresh weight, acetylene reduction activity (ARA), and plant DW among the eight genotypes examined. Stem starch concentration and milligram N per plant and nodule P concentration and ARA were also correlated. A strong correlation between the amount of N accumulated per milligram P and plant growth was shown. This supports an earlier suggestion that plant N concentration per plant P concentration may be a good indicator for N2 fixing ability at low P. E295 and ANT22 were the best, and G19833 the worst, of the genotypes in dry weight accumulation when P and N supply was limited. E295 had higher dry matter production early in the growth cycle; ANT22 produced more dry matter later in the growth cycle, and translocated a higher percentage of P and N to the seeds. ANT22 and E295 accumulated more total P than G19833 when grown under low-P conditions, and had greater P- and N-use efficiency, total N content (indicating higher N2 fixation), nodule P concentration, and nodule mass than G19833. G19833 contained less soluble carbohydrates in the stem than ANT22 and E295, but had a higher concentration in the roots. ANT22 and E295 appear more efficient than G19833 in taking up P from rock phosphate and/or organic sources when the plant is reliant on BNF. A large root/shoot ratio does not explain this difference.

Item Type: Article
Additional Information: Financial support for this research was provided by The Bean/Cowpea Collaborative Research Support Program.
Uncontrolled Keywords: Andean germplasm; Phaseolus vulgaris; Carbohydrate allocation; Low P tolerance; Nodulation; N2 fixation
Author Affiliation: Department of Soil, Water, and Climate, University of Minnesota, St. Paul, MN 55108, USA
Subjects: Crop Improvement
Divisions: Other Crops
Depositing User: Mr Siva Shankar
Date Deposited: 02 Aug 2013 10:13
Last Modified: 02 Aug 2013 10:13
Official URL:

Actions (login required)

View Item View Item