DNA polymorphisms in grain sorghum (Sorghum bicolor (L.) Moench)

Tao, Y. and Manners, J.M. and Ludlow , M.M. and Henzell , R.G. (1993) DNA polymorphisms in grain sorghum (Sorghum bicolor (L.) Moench). Theoretical and Applied Genetics , 86 (6). pp. 679-688.

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Molecular markers [random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP)] were used to determine the frequency of DNA polymorphism in grain sorghum (Sorghum bicolor (L.) Moench). Twenty-nine oligonucleotide primers were employed for RAPDs, generating a total of 262 DNA fragments, of which 145 were polymorphic in at least one pairwise comparison between 36 genotypes. Individual primers differed significantly in their ability to detect genetic polymorphism in the species. The overall frequency of polymorphisms was low with a mean frequency of 0.117 polymorphisms per RAPD band being obtained from all pairwise comparisons between genotypes, with maximum and minimum values of 0.212 and 0.039, respectively. Results from phenetic analysis of bandsharing data were consistent with current sub-specific groupings of the species, with clusters of Durra, Zerazera, Caud-Nig, Caud-Kaura and Caffrorum being discernible. The results also indicated that individuals of a similar taxonomic grouping but different geographic origin may be genetically less identical than previously considered. Similar frequencies of polymorphism to that obtained with RAPDs were obtained with RFLPs. Results from these experiments indicated that a high level of genetic uniformity exists within S. bicolor

Item Type: Article
Additional Information: SNNigam Collection
Uncontrolled Keywords: Random amplified polymorphic DNA,Restriction fragment length polymorphisms, Genetic diversity, Genome mapping, Sorghum (Sorghum bicolor)
Author Affiliation: CSIRO, Division of Tropical Crops and Pastures, Cunningham Laboratory, 306 Carmody Road, 4067, St. Lucia, Brisbane, Australia
Subjects: Crop Improvement
Divisions: Sorghum
Depositing User: Mr B Krishnamurthy
Date Deposited: 27 Sep 2013 06:41
Last Modified: 27 Sep 2013 06:41
Official URL: http://dx.doi.org/10.1007/BF00222656
URI: http://eprints.icrisat.ac.in/id/eprint/11872

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