Establishment of introgression libraries in hybrid rye (Secale cereale L.) from an Iranian primitive accession as a new tool for rye breeding and genomics

Falke, F.C. and Susic, Z. and Hackauf, B. and et al, . (2008) Establishment of introgression libraries in hybrid rye (Secale cereale L.) from an Iranian primitive accession as a new tool for rye breeding and genomics. Theoretical and Applied Genetics, 117 (4). pp. 641-652.

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Genetic diversity of elite breeding material can be increased by introgression of exotic germplasm to ensure long-term selection response. The objective of our study was to develop and characterize the first two rye introgression libraries generated by marker-assisted backcrossing and demonstrate their potential application for improving the baking quality of rye. Starting from a cross between inbred line L2053-N (recurrent parent) and a heterozygous Iranian primitive population Altevogt 14160 (donor) two backcross (BC) and three selfing generations were performed to establish introgression libraries A and B. Amplified fragment length polymorphisms (AFLP® markers) and simple sequences repeats (SSRs) were employed to select and characterize candidate introgression lines (pre-ILs) from BC1 to BC2S3. The two introgression libraries comprise each 40 BC2S3 pre-ILs. For analyzing the phenotypic effects of the exotic donor chromosome segment (DCS) we evaluated the per se performance for pentosan and starch content in replicated field trials at each of four locations in 2005 and 2006. Introgression library A and B cover 74 and 59% of the total donor genome, respectively. The pre-ILs contained mostly two to four homozygous DCS, with a mean length of 12.9 cM (A) and 10.0 cM (B). We detected eight (A) and nine (B) pre-ILs with a significant (P < 0.05) higher pentosan content and two pre-ILs (B) with a significant (P < 0.05) higher starch content than the elite recurrent parent. Thus, our results indicate that exotic genetic resources in rye carry favorable alleles for baking quality traits, which can be exploited for improving the elite breeding material by marker-assisted selection (MAS). These introgression libraries can substantially foster rye breeding programs and provide a promising opportunity to proceed towards functional genomics.

Item Type: Article
Additional Information: The present study was financially supported by by the German Federal Ministry of Education and Research, Bonn, the private plant breeding companies Hybro GmbH & Co. KG, Schenkendorf, and KWS LOCHOW GmbH, Bergen, in conjunction with the GABI program ‘‘Rye Resources’’ (BMBF Grant #0312289B) and the German Federal Ministry of Economics (BMWi) in the PRO INNO frame work (Aif Grant #KF0141101MD5).
Author Affiliation: State Plant Breeding Institute, Universitaet Hohenheim, 70593, Stuttgart, Germany
Subjects: Crop Improvement
Divisions: Other Crops
Depositing User: Mr Siva Shankar
Date Deposited: 30 Sep 2013 07:17
Last Modified: 30 Sep 2013 07:17
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