High temperature stress of Brassica napus during flowering reduces micro‐ and megagametophyte fertility, induces fruit abortion, and disrupts seed production

Young, L.W. and Wilen, R.W. and Bonham-Smith, P.C. (2004) High temperature stress of Brassica napus during flowering reduces micro‐ and megagametophyte fertility, induces fruit abortion, and disrupts seed production. Journal of Experimental Botany, 55 (396). pp. 485-495.

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High temperature stress (HTS), during flowering, decreases seed production in many plants. To determine the effect of a moderate HTS on flowering, fruit and seed set in Brassica napus, plants were exposed to a HTS (8/16 h dark/light, 18 °C night, ramped at 2 °C h–1, over 6 h, to 35 °C for 4 h, ramped at 2 °C h–1 back to 23 °C for 6 h) for 1 or 2 weeks after the initiation of flowering. Although flowering on the HTS‐treated plants, during both the 1 week and 2 week HTS treatments, was equal to that of control‐grown plants, fruit and seed development, as well as seed weight, were significantly reduced. Under HTS, flowers either developed into seedless, parthenocarpic fruit or aborted on the stem. At the cessation of the HTS, plants compensated for the lack of fruit and seed production by increasing the number of lateral inflorescences produced. During the HTS, pollen viability and germinability were slightly reduced. In vitro pollen tube growth at 35 °C, from both control pollen and pollen developed under a HTS, appeared abnormal, however, in vivo tube growth to the micropyle appeared normal. Reciprocal pollination of HTS or control pistils with HTS or control pollen indicated that the combined effects of HTS on both micro‐ and megagametophytes was required to knock out fruit and seed development. Expression profiles for a subset of HEAT SHOCK PROTEINs (HSP101, HSP70, HSP17.6) showed that both micro‐ and megagametophytes were thermosensitive despite HTS‐induced expression from these genes.

Item Type: Article
Additional Information: We would like to thank Fatma Kaplan from Charles Guy's laboratory (University of Florida) for designing and testing the 18S rRNA RT-PCR internal control primers and Andrew Sharpe and Derek Lydiate, Agriculture and AgriFood Canada, Saskatoon Research Centre for allowing us to search the B. napus EST database for the B. napus HSP sequences. Funding for this research was through the Canola Council of Canada, the Saskatchewan Canola Development Commission, and NSERC, Canada
Uncontrolled Keywords: Heat Shock Protein (HSP), high temperature stress, megagametophyte, microgametophyte, parthenocarpic, pollen.
Author Affiliation: Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, Saskatchewan S7N 5E2, Canada
Subjects: Atmosperic Science > Climatology
Plant Production
Crop Improvement
Divisions: General
Depositing User: Mr. SanatKumar Behera
Date Deposited: 04 Oct 2013 11:19
Last Modified: 04 Oct 2013 11:19
Official URL: http://dx.doi.org/10.1093/jxb/erh038
URI: http://eprints.icrisat.ac.in/id/eprint/11950

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