Genome sequencing reveals agronomically important loci in rice using MutMap

Abe, A. and Kosugi, S. and Yoshida, K. and et al, . (2012) Genome sequencing reveals agronomically important loci in rice using MutMap. Nature Biotechnology, 30. pp. 174-178.

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The majority of agronomic traits are controlled by multiple genes that cause minor phenotypic effects, making the identification of these genes difficult. Here we introduce MutMap, a method based on whole-genome resequencing of pooled DNA from a segregating population of plants that show a useful phenotype. In MutMap, a mutant is crossed directly to the original wild-type line and then selfed, allowing unequivocal segregation in second filial generation (F2) progeny of subtle phenotypic differences. This approach is particularly amenable to crop species because it minimizes the number of genetic crosses (n = 1 or 0) and mutant F2 progeny that are required. We applied MutMap to seven mutants of a Japanese elite rice cultivar and identified the unique genomic positions most probable to harbor mutations causing pale green leaves and semidwarfism, an agronomically relevant trait. These results show that MutMap can accelerate the genetic improvement of rice and other crop plants.

Item Type: Article
Author Affiliation: Iwate Agricultural Research Center, Kitakami, Japan.
Subjects: Crop Improvement > Biotechnology
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 16 Oct 2013 07:46
Last Modified: 16 Oct 2013 07:46
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