Gene discovery and functional marker development for fragrance in sorghum (Sorghum bicolor (L.) Moench)

Yundaeng, C. and Somta, P. and Tangphatsornruang, S. and et al, . (2013) Gene discovery and functional marker development for fragrance in sorghum (Sorghum bicolor (L.) Moench). Theoretical and Applied Genetics, 126 (11). pp. 2897-2906.

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2-acetyl-1-pyrroline (2AP) is a potent volatile compound causing fragrance in several plants and foods. Seeds of some varieties of rice, sorghum and soybean possess fragrance. The genes responsible for fragrance in rice and soybean are orthologs that correspond to betaine aldehyde dehydrogenase 2 (BADH2). Genotypes harboring fragrance in rice and soybean contain a premature stop codon in BADH2 which impairs the synthesis of full length functional BADH2 protein leading to the accumulation of 2AP. In this study, we reported an association between the BADH2 gene and fragrance in sorghum. An F2 population of 187 plants developed from a cross between KU630 (non-fragrant) and IS19912 (fragrant) was used. Leaves of F2 and F3 progenies were evaluated for fragrance by organoleptic test, while seeds of F2 plants were analyzed for 2AP. The tests consistently showed that the fragrance is controlled by a single recessive gene. Gene expression analysis of SbBADH1 and SbBADH2 in leaves of KU630 and IS19912 at various stages revealed that SbBADH1 and SbBADH2 were expressed in both accessions. Sequence comparison between KU630 and IS19912 revealed a continuous 1,444 bp deletion encompassing exon 12 to 15 of SbBADH2 in IS19912 which introduces a frameshift mutation and thus causes a premature stop codon. An indel marker was developed to detect polymorphism in SbBADH2. Bulk segregant and QTL analyses confirmed the association between SbBADH2 and fragrance.

Item Type: Article
Additional Information: This research was supported by the Royal Golden Jubilee Ph.D. Scholarship co-funded by the Thailand Research Fund (TRF) and Kasetsar University to P. Somta and C. Yundaeng.
Author Affiliation: Program in Plant Breeding, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, 73140, Thailand
Subjects: Crop Improvement > Genetics/Genomics
Divisions: Sorghum
Depositing User: Mr Siva Shankar
Date Deposited: 28 Oct 2013 06:03
Last Modified: 28 Oct 2013 06:03
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