Identification, phylogeny, and transcript profiling of ERF family genes during development and abiotic stress treatments in tomato

Sharma, M.K. and Kumar, R. and Solanke, A.U. and et al, . (2010) Identification, phylogeny, and transcript profiling of ERF family genes during development and abiotic stress treatments in tomato. Molecular Genetics and Genomics , 284 (6). pp. 455-475.

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Abstract

Ethylene responsive transcription factors have been shown to be intimately connected to plant development, defense responses and stress signaling pathways and in order to use them for plant improvement, we need to have better understanding of these proteins. In this study, 85 ERF genes have been identified from tomato using raw EST data in various public repositories. Phylogenetic analysis with tomato ERF domains revealed their distribution in all the groups, previously identified in model systems. MEME motif analysis resulted in identification of conserved domains, characteristic to member of each clade, in addition to ERF domain. Expression analysis during vegetative and reproductive stages of development using QPCR and tomato GeneChip® arrays, revealed their tissue-specific/preferential accumulation. In total, 57 genes were found to be differentially expressed during temporal stages of tomato fruit development. The expression analysis of 23 ERF family genes representing each clade in response to seven abiotic stress treatments revealed their differential expression in response to more than one abiotic stress treatments. Results suggest that ERF genes play diverse roles in plant’s life and comprehensive data generated will be helpful in conducting functional genomics studies to understand their precise role during plant development and stress response

Item Type: Article
Uncontrolled Keywords: Development Ethylene, Ethyleneresponse factor, Expression, Ripening, Stress
Author Affiliation: Department of Plant Molecular Biology, University of Delhi (South Campus), New Delhi, 110021, India
Subjects: Crop Improvement
Plant Physiology and Biochemistry > Biochemistry
Divisions: Other Crops
Depositing User: Mr Daneti Raju
Date Deposited: 26 Jan 2014 12:05
Last Modified: 26 Jan 2014 12:05
Official URL: http://dx.doi.org/10.1007/s00438-010-0580-1
URI: http://eprints.icrisat.ac.in/id/eprint/12390

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