Genetic diversity in 'ex-situ' conserved sorghum accessions of Botswana as estimated by microsatellite markers

Motlhaodi, T. and Geleta, M. and Bryngelsson, T. and et al, . (2014) Genetic diversity in 'ex-situ' conserved sorghum accessions of Botswana as estimated by microsatellite markers. Australian Journal of Crop Science, 8 (1). pp. 35-43.

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Simple sequence repeat (SSR) markers were used to characterize genetic diversity in 30 sorghum [Sorghum bicolor (L.) Moench] accessions conserved in the national gene bank of Botswana. This collection represents the three sorghum-growing agro-ecological zones in the country. Ten microsatellite primer pairs were used, and these generated a total of 53 alleles with three to seventeen alleles per locus across the 30 accessions. A high level of genetic variation was observed among the accessions (70% of the variation), and 30% of the total genetic variation was observed within accessions. The analysis of molecular variance on sorghum accessions grouped based on agro-ecological region, ethnicity, and sorghum race revealed a non-significant variation, indicating that the alleles are distributed all over the country. Cluster analysis also did not group the accessions according to agro-ecological region, race or ethnicity. The results of this study have indicated that substantial genetic diversity exists among sorghum accessions conserved in Botswana, but that agro-ecological region, ethnicity and race did not influence the distribution of this diversity

Item Type: Article
Uncontrolled Keywords: accessions, genetic diversity, simple sequence repeats, sorghum
Author Affiliation: Swedish University of Agricultural Sciences, Department of Plant Breeding, P. O. Box 101, SE-230 53 Alnarp, Sweden
Subjects: Crop Improvement
Divisions: Sorghum
Depositing User: Mr B Krishnamurthy
Date Deposited: 23 Feb 2014 08:49
Last Modified: 23 Feb 2014 08:49
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