Gene expression profiling and identification of resistance genes to low temperature in leaves of peanut (Arachis hypogaea L.)

Chen, N. and Yang, Q. and Hu, D. and et al, . (2014) Gene expression profiling and identification of resistance genes to low temperature in leaves of peanut (Arachis hypogaea L.). Scientia Horticulturae, 169. pp. 214-225.

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Abstract

The cultivated peanut (Arachis hypogaea L.) is important oil crop and cold stress seriously influences its development and yields. Tolerant varieties produced using transgenic techniques will effectively increase peanut plantation area and enhance yields. However, little is known about the network of gene expression regulation related to cold stress in peanut. Therefore, we screened genes regulated by cold stress in peanut. In total, 1478 up-regulated and 1510 down-regulated probe sets were successfully identified in leaves of peanut subjected to cold stress using the microarray technique. Data analysis indicated that the main biological processes involved in cold stress response included signal transduction, transcript regulations and accumulation of soluble metabolites. Regulation of cell division, translation, protein modification, transport and DNA/RNA-related processes were also involved in peanut cold acclimation. Our study also revealed that protein kinases, transcription factors, heat shock proteins, resistance proteins, stilbene synthase, and so on, may play important functions in cold stress regulation of peanut. The function of some probe sets in cold stress regulation had not been clarified (e.g. proteins functioning in glycerol ether metabolic process, microtubule-based movement or nutrient reservoir activity). Some genes we identified lack functional annotation and their roles in response to cold are yet to be elucidated. These results showed an overview of the transcription map of peanut under cold stress, which may yield some useful insights into cold-mediated signal transduction pathways in peanut and offer some candidate genes as potential markers of tolerance to cold stress.

Item Type: Article
Additional Information: This study was supported by grants from China Agriculture Research System (CARS-14), the National Natural Science Founda- tion of China (31000728; 31100205; 31200211), the Natural Science Fund of Shangdong Province (ZR2011CQ036; ZR2012CQ031), the Promotive Research Fund for Young and Middle-aged Scientisits of Shandong Province (BS2010NY023), Qingdao Municipal Science and Technology Plan Project (11-2-4-9-(3)-jch; 11-2-3-26-nsh; 12- 1-4-11-(2)-jch). Supplementary Data: Supplementary data associated with this article can be found, in the online version, at http://dx.doi.org/10.1016/j.scienta.2014. 01.043.
Uncontrolled Keywords: Gene expression profiling; Microarray; Cold stress; Peanut (Arachis hypogaea L.)
Author Affiliation: Shandong Peanut Research Institute, Qingdao 266100, PR China
Subjects: Crop Improvement
Divisions: Groundnut
Depositing User: Mr Siva Shankar
Date Deposited: 20 Mar 2014 05:51
Last Modified: 20 Mar 2014 05:51
Official URL: http://dx.doi.org/10.1016/j.scienta.2014.01.043
URI: http://eprints.icrisat.ac.in/id/eprint/12920

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