Transgenic resistance by N gene of a Peanut bud necrosis virus isolate of characteristic phylogeny

Venkatesan, S. and Raja, J.A.J. and Maruthasalam, S. and et al, . (2009) Transgenic resistance by N gene of a Peanut bud necrosis virus isolate of characteristic phylogeny. Virus Genes, 38 (3). pp. 445-454.

[img] PDF - Published Version
Restricted to ICRISAT researchers only


The nucleocapsid protein (N) gene of a Tospovirus devastating tomato crop in the south Indian state of Tamil Nadu was cloned and characterized. The high identity of the cloned sequence to a Peanut bud necrosis virus (PBNV) tomato isolate (97.8/99.6% nucleotide/amino acid) and a PBNV peanut isolate (94.4/96.3% nucleotide/amino acid) identified the Tospovirus as an isolate of PBNV, designated PBNV Coimbatore tomato (PBNV CT) isolate. Phylogenetic analysis of PBNV CT N gene provided useful insights into the movement and evolution of PBNV within Indian Territory. The characteristic phylogeny of PBNV CT N gene implied its potential to be an efficient transgene to confer effective PBNV resistance on crop plants. The efficacy of PBNV CT N gene in conferring PBNV resistance was studied by generating tobacco (Nicotiana tabacum L. cv Wisconsin) lines transgenic to the sense or antisense version of the gene. Several transgenic lines showed transgenic mRNA and/or protein accumulation, ranging from very high to undetectable levels, accompanied by different degrees of PBNV resistance. The undetectable or very low levels of transgene transcripts in certain PBNV-resistant sense or antisense N gene transgenic lines suggested RNA-mediated resistance by post-transcriptional gene silencing (PTGS) mechanism. However, PBNV resistance of certain transgenic lines with high levels of N gene transcripts was suggestive of possible operation of RNA-mediated non-PTGS mechanism(s) of resistance in those lines. Moreover, the high levels of N protein in certain PBNV-resistant sense N gene transgenic lines suggested protein-mediated resistance. The results predict the potential of PBNV CT N gene to confer effective PBNV resistance on tomato and other economically important crops

Item Type: Article
Additional Information: Dr. P. Balasubramanian acknowledges the Department of Science and Technology (DST), India, for the financial assistance. The authors acknowledge the help provided by Dr. R. Usha, School of Biotechnology, Madurai Kamaraj University, Tamil Nadu, India and Dr. P. Lavakumar, ICRISAT, Andhra Pradesh, India.
Uncontrolled Keywords: Peanut bud necrosis virus, N gene, Pathogen-derived resistance
Author Affiliation: Tamil Nadu Agricultural University, Coimbatore
Subjects: Soil Science and Microbiology > Microbiology
Crop Improvement
Divisions: Groundnut
Depositing User: Ms Ishrath Durafsha
Date Deposited: 19 May 2014 09:10
Last Modified: 19 May 2014 09:10
Official URL:

Actions (login required)

View Item View Item