The effect of colchicine pretreatment on isolated microspore culture of wheat (Triticum aestivum L.)

Islam, S.M.S. (2010) The effect of colchicine pretreatment on isolated microspore culture of wheat (Triticum aestivum L.). Australian Journal of Crop Science, 4 (9). pp. 660-665.

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Colchicine is an alkaloid and microtubule-depolymerizing agent, disrupts microtubule formation during the cell division at metaphase stage and prevents anaphase thus resulting in an unreduced chromosome number before cell division. The main advantageous for genetic studies and plant breeding programmes using colchicine for rapid and huge production of fertile plants through anther and microspore culture. Therefore, the aim of this investigation was to find out the impact of colchicine on the response of fertile plants in wheat isolated microspore culture. In this case colchicine applied to the anthers compared with direct treatment on the isolated microspores. For first experiment 100 mg/l colchicine added seperately with three different basal media and found in all cases more or less fertile plants production compared to control (without colchicine). Colchicine application on anther culture medium showed significant increase in embryo formation and green plant regeneration. The direct treatment of colchicine to isolated microspore culture decreased three - four fold embryoids induction but improved fertile plant regeneration. Application of colchicine for both cases in anther and microspore culture increased the average chromosome doubling frequency (84.94%) compared to the control (55.26%) where colchicine was not added. For the second experiment, different concentrations of colchicine (50, 100, 150 mg/l for three days) added in AMC medium and found that reduced number of embryos and regenerated green plants, while the regeneration of albino plants was stimulated. When colchicine concentration increased the number of embryos decreased significantly, while the doubling index increased which is the main target of this study. In this case average frequency of diploid plants were increased (81.73%) compared with control (72.40%) among the three treatments. This finding has increased the knowledge about the benefit of colchicine application and optimized its concentration on isolated microspore for improving doubled haploids production in wheat microspore culture

Item Type: Article
Author Affiliation: Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh
Subjects: Crop Improvement > Biotechnology
Divisions: Other Crops
Depositing User: David T
Date Deposited: 09 Jan 2011 22:24
Last Modified: 09 Jan 2011 22:26
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