Production of Haploids and Doubled Haploids in Maize

Prigge, V. and Melchinger, A.E. (2012) Production of Haploids and Doubled Haploids in Maize. In: Plant Cell Culture Protocols. Humana Press Inc., New York, pp. 161-172. ISBN 978-1-61779-818-4

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The in vivo haploid induction approach offers several advantages compared to the in vitro induction approach and recurrent self-pollination. It is currently the method of choice for inbred line development in many commercial maize breeding programs. Here, we describe the in vivo approach for generation of maternal doubled haploids (DHs). It involves four steps: (1) induction of haploidy by pollinating source germplasm with pollen of a haploid inducer; (2) identification of putative haploid seeds (seeds with a haploid embryo) using a seed coloration marker system; (3) doubling of chromosomes of putative haploids by treating seedlings with a mitotic inhibitor; and (4) verification of putative doubled haploids with a stalk color marker and self-pollination of true doubled haploid plants to multiply their seed.

Item Type: Book Section
Additional Information: The scientifi c and editorial contributions of B. S. Dhillon, W. Schipprack, B. Devezi Savula, and H. H. Geiger are gratefully acknowledged. Vanessa Prigge is indebted to the Eiselen Foundation Ulm, Germany, for fi nancial support of her work as a joint PhD student between CIMMYT and the University of Hohenheim, Germany.
Uncontrolled Keywords: Doubled haploid, Chromosome doubling, Colchicine, In vivo haploid induction, Haploid, Maize
Author Affiliation: Institute of Plant Breeding, Seed Science, and Population Genetics, University of Hohenheim, Stuttgart, Germany
Subjects: Crop Improvement
Divisions: Maize
Depositing User: Mr Siva Shankar
Date Deposited: 20 May 2014 08:31
Last Modified: 20 May 2014 08:31

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