Role of catalase, H2O2 and phenolics in resistance of pigeonpea towards Helicoverpa armigera (Hubner)

Kaur, R. and Gupta, A.K. and Taggar, G.K. (2014) Role of catalase, H2O2 and phenolics in resistance of pigeonpea towards Helicoverpa armigera (Hubner). Acta Physiologiae Plantarum, 36 (6). pp. 1513-1527.

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Rapid generation of superoxide radicals and accumulation of H2O2 is a characteristic early response of plants following perception of insect herbivory signals. Induction of oxidative burst on account of herbivory triggers various defense mechanisms in plants. Response of superoxide and H2O2-metabolizing enzymes and secondary metabolites in nine pigeonpea genotypes to Helicoverpa armigera feeding was investigated. Out of nine, four genotypes were found to be moderately resistant, three were intermediate and two were moderately susceptible. In general, H. armigera infestation resulted in increase in superoxide dismutase activity, H2O2 and phenolics content and decrease in catalase (CAT) activity in leaves, developing seeds and pod wall of pigeonpea genotypes. Peroxidase activity was found only in leaves. Among genotypes, the increase in phenolic constituents was found greater in moderately resistant genotypes than in moderately susceptible genotypes; this might determine their contribution in providing resistance to genotypes against H. armigera infestation. The capability of moderately resistant genotypes to maintain relatively lower H2O2 content and higher CAT activity in pod wall and developing seeds also appeared to determine resistance of genotypes towards H. armigera. Expression of resistance to H. armigera was found to be associated with a negative correlation of H2O2-metabolizing enzymes and phenolics with pod damage as well as with negative association between CAT activity and H2O2 content. A positive correlation found between H2O2 content and pod damage suggested the accumulation of H2O2 in response to pod borer attack. In addition, correlation analysis also revealed a positive association between CAT, phenolic compounds and DPPH radical scavenging activity following pod borer attack; this indicated their contribution in resistance mechanisms against H. armigera herbivory.

Item Type: Article
Uncontrolled Keywords: Oxidative burst, Pigeonpea, Helicoverpa armigera, H2O2-metabolizing enzymes, Secondary, metabolites, H2O2
Author Affiliation: Department of Biochemistry, Punjab Agricultural University, Ludhiana, 141004, India
Subjects: Crop Improvement
Plant Physiology and Biochemistry > Plant Physiology
Divisions: Pigeonpea
Depositing User: Mr Siva Shankar
Date Deposited: 22 May 2014 10:34
Last Modified: 22 May 2014 10:34
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