Deposition, turnover, and release of CENH3 at Arabidopsis centromeres

Lermontova, I. and Rutten, T. and Schubert, I. (2011) Deposition, turnover, and release of CENH3 at Arabidopsis centromeres. Chromosoma , 120 (6). pp. 633-640.

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The kinetochore is a complex multiprotein structure located at centromeres and required for the proper segregation of chromosomes during mitosis and meiosis. An important role in kinetochore assembly and function plays the centromeric histone H3 variant (CENH3). Cell cycle stage of CENH3 deposition to centromeres varies between different organisms. We confirmed by in vivo studies that deposition of Arabidopsis CENH3 takes place at centromeres during G2 and demonstrated that additionally a low turnover of CENH3 occurs along the cell cycle, apparently for replacement of damaged protein. Furthermore, enhanced yellow fluorescent protein (EYFP)-CENH3 of photobleached chromocenters is not replaced by EYFP-CENH3 molecules from unbleached centromeres of the same nucleus, indicating a stable incorporation of CENH3 into centromeric nucleosomes. In differentiated endopolyploid nuclei however, the amount of CENH3 at centromeres declines with age

Item Type: Article
Additional Information: This work was supported by a grant from the Deutsche Forschungsgemeinschaft to I.L. (LE2299/1-1).
Uncontrolled Keywords: Centromeres, CENH3, Yeast cells, Chicken, Metaphase
Author Affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Gatersleben, Germany
Subjects: Crop Improvement > Genetics/Genomics
Plant Physiology and Biochemistry > Biochemistry
Divisions: General
Depositing User: Ms Ishrath Durafsha
Date Deposited: 17 Jun 2014 09:02
Last Modified: 17 Jun 2014 09:02
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