Determination of cyanogenic glucosides in white clover (Trifolium repens L.) by high performance liquid chromatography

Stochmal, A. and Oleszek, W. (1994) Determination of cyanogenic glucosides in white clover (Trifolium repens L.) by high performance liquid chromatography. Phytochemical Analysis, 5 (5). pp. 271-272.

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Abstract

A high performance liquid chromatographic method used for the determination of cyanogenic glucosides in flax seed has been modified to make it suitable for the analysis of these compounds in dried white clover (Trifolium repens L.) samples. Moreover, the extraction of glycosides from clover samples and purification of exracts on the C18 support prior to analysis has been developed. Linamarin and lotaustralin were identified in T. repens cv. Armena in concentrations of 1.29 and 3.13 mg/g of dry matter, respectively

Item Type: Article
Additional Information: We thank Dr B. D. Oomah from Agriculture Canada Research Station, Modern, Manitoba for supplying the linamarin standard.
Uncontrolled Keywords: High performance liquid chromatography; Linamarin; Lotaustralin; Quantitative analysis
Author Affiliation: Department of Biochemistry, Institute of Soil Science and Plant Cultivation, Osada Palacowa, 24-100 Pulawy, Poland
Subjects: Plant Production
Plant Physiology and Biochemistry > Plant Physiology
Social Sciences > Plant Physiology and Biochemistry > Plant Physiology

Plant Physiology and Biochemistry > Biochemistry
Social Sciences > Plant Physiology and Biochemistry > Biochemistry
Divisions: Other Crops
Depositing User: Ms Ishrath Durafsha
Date Deposited: 25 Jun 2014 04:28
Last Modified: 25 Jun 2014 04:28
Official URL: http://dx.doi.org/10.1002/pca.2800050511
URI: http://eprints.icrisat.ac.in/id/eprint/13276

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