Technical approaches to inoculate micropropagated sugar cane plants were Acetobacter diazotrophicus

Reis, V.M. and Olivares, F.L. and Oliveira, A.L.M. de and et al, . (1999) Technical approaches to inoculate micropropagated sugar cane plants were Acetobacter diazotrophicus. Plant and Soil, 206 (2). pp. 205-211.

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Micropropagated plantlets of sugar cane were inoculated with the N2-fixing bacterium Acetobacter diazotrophicus. Various modifications on the basic plant culture medium MS were made for the plant/bacteria association. The protocol required the inoculation of the bacteria at the end of the rooting period in a medium without hormones or vitamins, and with the concentration of sugar and mineral nutrients reduced by a factor of 10. Individual plants were inoculated with A. diazotrophicus and maintained under the appropriate light and temperature condition used for micropropagation up to 7 days. The system favored the infection and the establishment of the bacteria within the plant tissue. Bacteria colonized the plant tissue and accumulated in inter-cellular cavities and the region of lateral root emergence and also colonizes the xylem vessels. The inoculated plantlets were subsequently transferred to the acclimatization phase and after 30 days it was possible to isolate the bacteria from plant tissue. This protocol permitted studies of infection and comparison among strains

Item Type: Article
Uncontrolled Keywords: Acetobacter diazotrophicus, endophytic diazotrophic bacteria, micropropagated plants, nitrogen fixation, sugar cane, tissue culture
Author Affiliation: Embrapa Agrobiologia, km 47, Seropédica, RJ, CP 74.505, CEP 23851-970, Brazil
Subjects: Plant Protection
Divisions: Other Crops
Depositing User: Ms K Syamalamba
Date Deposited: 11 Jul 2014 07:48
Last Modified: 11 Jul 2014 07:48
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