Hybrid recreation by reverse breeding in arabidopsis thaliana

Wijnker, E. and Deurhof, L. and Belt, J. van de and et al, . (2014) Hybrid recreation by reverse breeding in arabidopsis thaliana. Nature Protocols, 9 (4). pp. 761-772.

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Hybrid crop varieties are traditionally produced by selecting and crossing parental lines to evaluate hybrid performance. Reverse breeding allows doing the opposite: selecting uncharacterized heterozygotes and generating parental lines from them. With these, the selected heterozygotes can be recreated as F1 hybrids, greatly increasing the number of hybrids that can be screened in breeding programs. Key to reverse breeding is the suppression of meiotic crossovers in a hybrid plant to ensure the transmission of nonrecombinant chromosomes to haploid gametes. These gametes are subsequently regenerated as doubled-haploid (DH) offspring. Each DH carries combinations of its parental chromosomes, and complementing pairs can be crossed to reconstitute the initial hybrid. Achiasmatic meiosis and haploid generation result in uncommon phenotypes among offspring owing to chromosome number variation. We describe how these features can be dealt with during a reverse-breeding experiment, which can be completed in six generations (~1 year)

Item Type: Article
Author Affiliation: Laboratory of Genetics, Wageningen University, Wageningen, The Netherlands
Subjects: Crop Improvement
Divisions: Other Crops
Depositing User: Ms K Syamalamba
Date Deposited: 22 Sep 2014 09:15
Last Modified: 22 Sep 2014 09:28
Official URL: http://dx.doi.org/doi:10.1038/nprot.2014.049
URI: http://eprints.icrisat.ac.in/id/eprint/13442

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