Fine-mapping of qRfg2, a QTL for resistance to Gibberella stalk rot in maize

Zhang, D. and Liu, Y. and Guo, Y. and et al, . (2012) Fine-mapping of qRfg2, a QTL for resistance to Gibberella stalk rot in maize. Theor Appl Genet, 124. pp. 585-596.

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Stalk rot is one of the most devastating diseases in maize worldwide. In our previous study, two QTLs, a major qRfg1 and a minor qRfg2, were identified in the resistant inbred line ‘1145’ to confer resistance to Gibberella stalk rot. In the present study, we report on finemapping of the minor qRfg2 that is located on chromosome 1 and account for *8.9% of the total phenotypic variation. A total of 22 markers were developed in the qRfg2 region to resolve recombinants. The progeny-test mapping strategy was developed to accurately determine the phenotypes of all recombinants for fine-mapping of the qRfg2 locus. This finemapping process was performed from BC4F1 to BC8F1 generations to narrow down the qRfg2 locus into *300 kb, flanked by the markers SSRZ319 and CAPSZ459. A predicted gene in the mapped region, coding for an auxin-regulated protein, is believed to be a candidate for qRfg2. The qRfg2 locus could steadily increase the resistance percentage by *12% across different backcross generations, suggesting its usefulness in enhancing maize resistance against Gibberella stalk rot.

Item Type: Article
Author Affiliation: D. ZhangNational Maize Improvement Center of China, China Agricultural University, 2 West Yuanmingyuan Road, Beijing 100193, People’s Republic of China
Subjects: Plant Production
Divisions: Maize
Depositing User: Mr Daneti Raju
Date Deposited: 06 Feb 2015 10:32
Last Modified: 06 Feb 2015 10:32
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