Multidimensional Drug Profiling by Automated Microscopy

Perlman, Z.E. and Slack, M.D. and Feng, Y. and et al, . (2004) Multidimensional Drug Profiling by Automated Microscopy. Science, 306 (5699). pp. 1194-1198.

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We present a method for high-throughput cytological profiling by microscopy. Our system provides quantitative multidimensional measures of individual cell states over wide ranges of perturbations. We profile dose-dependent phenotypic effects of drugs in human cell culture with a titration-invariant similarity score (TISS). This method successfully categorized blinded drugs and suggested targets for drugs of uncertain mechanism. Multivariate single-cell analysis is a starting point for identifying relationships among drug effects at a systems level and a step toward phenotypic profiling at the single-cell level. Our methods will be useful for discovering the mechanism and predicting the toxicity of new drugs

Item Type: Article
Uncontrolled Keywords: Phenotype, Multidimensional profiling methods, Transcript analysis, Molecular cytology
Author Affiliation: Institute of Chemistry and Cell Biology, Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
Subjects: Soil Science and Microbiology > Microbiology
Crop Improvement > Biotechnology
Plant Physiology and Biochemistry > Biochemistry
Divisions: General
Depositing User: Ms Ishrath Durafsha
Date Deposited: 13 Nov 2014 04:12
Last Modified: 13 Nov 2014 04:12
Official URL:

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