Characterization of the large (L) RNA of peanut bud necrosis tospovirus

Gowda, S. and Satyanarayana, T. and Naidu, R.A. and Mushegian, A. and Dawson, W.O. and Reddy, D.V.R. (1998) Characterization of the large (L) RNA of peanut bud necrosis tospovirus. Archives of Virology, 143 (12). pp. 2381-2390.

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The nucleocapsids purified from groundnut plants systemically infected with peanut bud necrosis tospovirus (PBNV) contained both viral (v) and viral complementary (vc) sense L RNAs. Defective forms of L RNA containing core polymerase region were observed. The full length L RNA of PBNV was sequenced using overlapping cDNA clones. The 8911 nucleotide L RNA contains a single open reading frame (ORF) in the vc strand, and encodes a protein of 330 kDa. At the 5′ and 3′ termini of the v sense RNA there were 247 and 32 nt untranslated regions, respectively, containing an 18 nt complementary sequence with 1 mismatch. Comparisons of the predicted amino acid sequence of the L protein of PBNV with other members of Bunyaviridae suggest that the L protein of PBNV is a viral polymerase. The L protein had highest identity in the core-polymerase domain with the corresponding regions of tospoviruses, tomato spotted wilt tospovirus and impatiens necrotic spot tospovirus

Item Type: Article
Author Affiliation: The University of Florida Citrus Research and Education Center, Lake Alfred, FL, USA
Subjects: Plant Protection > Pests
Crop Improvement
Divisions: Groundnut
Depositing User: Mr B Krishnamurthy
Date Deposited: 16 Jan 2011 21:10
Last Modified: 16 Jan 2011 21:10
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