Enhanced tolerance to heat stress in transgenic plants expressing the GASA4 gene

Ko, Chang-Beom and Woo, Young-Min and Lee, Dong Ju and Lee, Myung-Chul and Kim, Cheol Soo (2007) Enhanced tolerance to heat stress in transgenic plants expressing the GASA4 gene. Plant Physiology and Biochemistry, 45 (9). pp. 722-728.

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Abstract

We conducted a genetic yeast screen to identify Thermo-tolerance genes (TTOs) in maize kernel cDNA library. During the screening, we identified a maize clone (TTO6) that seemed to confer elevated heat tolerance in comparison to control cells. TTO6 cDNA (GenBank accession no. AY103785) encodes an 11-kDa protein which is 69% similarity to the Arabidopsis GASA4 gene. To further examine heat tolerance in Arabidopsis, we functionally characterized the GASA4 gene and found that heat induced GASA4 expression. Constitutive expression of GASA4 in Arabidopsis led to elevated heat tolerance in transgenic lines. Interestingly, endoplasmic reticulum chaperone expression analysis suggeststhat GASA4 influences BiP gene expression during heat stress.

Item Type: Article
Author Affiliation: Department of Plant Biotechnology and Agricultural Plant Stress Research Center, Chonnam National University, 300 Yongbong-dong, Buk-gu, Kwangju 500-757, South Korea
Subjects: Plant Production
Plant Physiology and Biochemistry > Biochemistry
Social Sciences > Plant Physiology and Biochemistry > Biochemistry
Divisions: Maize
Depositing User: Sandhya Gir
Date Deposited: 20 Jan 2011 17:46
Last Modified: 20 Jan 2011 17:46
Official URL: http://dx.doi.org/10.1016/j.plaphy.2007.07.010
URI: http://eprints.icrisat.ac.in/id/eprint/1413

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