Agrobacterium - mediated transformation of maize

Ishida, Yuji and Hiei, Yukoh and Komari, Toshihiko (2007) Agrobacterium - mediated transformation of maize. Nature Protocols, 2 (7). pp. 1614-1621.

[img] PDF
Restricted to ICRISAT researchers only

Abstract

Maize may be transformed very efficiently using Agrobacterium tumefaciens-mediated methods. The most critical factor in the transformation protocol is the co-cultivation of healthy immature embryos of the correct developmental stage with A. tumefaciens; the embryos should be collected only from vigorous plants grown in well-conditioned glasshouses. With the protocol described here, approximately 50% of immature embryos from the inbred line A188 and 15% from inbred lines A634, H99 and W117 will produce transformants. About half of the transformed plants are expected to carry one or two copies of the transgenes, which are inherited by the progeny in a mendelian fashion. More than 90% of transformants are expected to be normal in morphology. The protocol takes about 3 months from the start of co-cultivation to the planting of transformants into pots.

Item Type: Article
Author Affiliation: Plant Innovation Center, Japan Tobacco Inc., 700 Higashibara, Iwata, Shizuoka 438-0802, Japan
Subjects: Crop Improvement > Genetics/Genomics
Divisions: Maize
Depositing User: Sandhya Gir
Date Deposited: 23 Jan 2011 16:06
Last Modified: 23 Jan 2011 16:06
Official URL: http://www.nature.com/nprot/journal/v2/n7/full/npr...
URI: http://eprints.icrisat.ac.in/id/eprint/1419

Actions (login required)

View Item View Item