An effective method of sonication-assisted Agrobacterium-mediated transformation of chickpeas

Pathak, M.R. and Harnzah, R.Y. (2008) An effective method of sonication-assisted Agrobacterium-mediated transformation of chickpeas. Plant Cell, Tissue and Organ Culture, 93 (1). pp. 65-71.

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An efficient and reproducible transformation method of sonication- assisted Agrobacteriummediated transformation (SAAT) was developed for chickpea (Cicer arietinum L.). Agrobacterium tumefaciens (LBA4404) harboring pCAMBIA1305.2 was used to transform decapitated embryo explants of two cultivars of chickpeas. By using a series of co-cultivation, callus induction, shoot initiation and root inducing media, a large number of transgenic plants were recovered. Transient expressions of GUS gene were detected by X-Gluc histochemical assay in transformed tissues. DNA analysis of TO and Tl· plants by PCR and Southern hybridization confirmed the integration of transgenes in initial and next generation transform ants in different transgenic lines. The transformation efficiency was more than two times higher in SAAT treatment than simple Agrobacterium without sonication.

Item Type: Article
Uncontrolled Keywords: Agrobacterium tumefaciens, Chickpeas, SAAT, Transformation
Author Affiliation: Biotechnology Program, Arabian Gulf University, P.O.Box 26671, Manama, Kingdom of Bahrain
Subjects: Plant Protection
Divisions: Chickpea
Depositing User: Mr B Krishnamurthy
Date Deposited: 03 Feb 2017 04:20
Last Modified: 03 Feb 2017 04:20
Official URL: 007/s I 1240-008-9344-6

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