Comparative analysis of ESTs in response to drought stress in chickpea (C. arietinum L.)

Gao, W.R. and Wang, X.S. and Liu, Q.U. and et al, . (2008) Comparative analysis of ESTs in response to drought stress in chickpea (C. arietinum L.). Biochemical and Biophysical Research Communications, 376. pp. 578-583.

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Chickpea (Cicer arietinum L.) is an important pulse crop grown mainly in the arid and semi-arid regions. To identify the water-stress-induced genes, two non-normalized cDNA libraries were constructed from the seedling leaves of a drought-tolerant chickpea cultivar under PEG-treated and -nontreated conditions. About 2500 clones from each library were selected randomly for sequencing analysis. Based on IDEG6 online software analysis, 92 genes were differentially expressed, and these genes were involved in diverse biological progresses, such as metabolism, transcription, signal transduction, protein synthesis and others. Most of the up-regulated genes were related to drought tolerance, and the down-regulated genes were mainly involved in photosynthesis. The differential expression patterns of five functional unigenes were confirmed by quantitative real-time PCR (qPCR). The results will help in understanding the molecular basis of drought tolerance in chickpea.

Item Type: Article
Uncontrolled Keywords: Chickpea, Drought stress, Drought tolerance, cDNA library, Expressed sequence tags, Quantitative real-time PCR
Author Affiliation: State Key Lab of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Department of Agriculture, Nanjing Agricultural University, Xuanwu District, Weigang No. 1, Nanjing 210095, China
Subjects: Plant Protection
Divisions: Chickpea
Depositing User: Mr B Krishnamurthy
Date Deposited: 03 Feb 2017 04:22
Last Modified: 03 Feb 2017 04:22
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