Botanicals and the growth of Aspergillus flavus from stored Groundnuts

Okereke, V.C. and Godwin-Egein, M.I. and Oji, C.T. (2017) Botanicals and the growth of Aspergillus flavus from stored Groundnuts. World Journal of Agricultural Sciences, 13 (1). pp. 39-44.

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Sampling of stored groundnut was carried out in five different markets in Port Harcourt, Nigeria to determine the occurrence of mycotoxin producing fungi and the subsequent effects of some botanicals on most prevalent fungus in vitro. The fungi were isolated and identified by standard methods of culturing and subculturing. Aqueous leaf extracts were obtained from locally sourced Garcinia kola, Gongronema latifolium, Moringer oleifera, Azadirachta indica, Ocimium gratissimum and Vernonia amygdalina. The extracts were applied at three different concentrations namely; 25, 50 and 75g per litre in sterile distilled water. The experiment was laid out in completely randomized design (CRD) replicated four times. Results showed that Aspergillus flavus, Aspergillus niger and Fusarium oxysporum were predominantly isolated from the grains, with A. flavus having the highest occurrence. Mycelial growth of Aspergillus flavus was significantly (P<0.001) reduced by V. amygdalina, G. kola, O. gratissimium and G. latifolium in ascending order of magnitude, at 7 days after treatment. The concentration of 50g/litre showed the best inhibitory effects. V. amygdalina, G. kola, O. gratissimium and G. latifolium @ 50g/litre could form an integral part of active component for botanical based fungicide production to protect stored groundnuts against Aspergillus flavus and subsequent aflatoxin contamination.

Item Type: Article
Uncontrolled Keywords: Groundnut Leaf extract Aspergillus flavus Mycelial growth Concentration
Author Affiliation: Department of Crop and Soil Science, Faculty of Agriculture, University of Port Harcourt, PMB 5323, Port Harcourt, Nigeria
Subjects: Crop Improvement
Divisions: Groundnut
Depositing User: Mr T L Gautham
Date Deposited: 21 Jul 2017 03:13
Last Modified: 21 Jul 2017 03:13

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