Interspecific hybridization between Cicer arietinum and C. pinnatifidum

Badami, P.S. and Mallikarjuna, N. and Moss, J.P. (1997) Interspecific hybridization between Cicer arietinum and C. pinnatifidum. Plant Breeding, 116 (4). pp. 393-395.

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An interspecific hybrid between Cicer arietinum cv. GL769 and a wild species C. pinnatifidum was obtained after emasculation, pollination and application of growth regulators. Ovules were cultured and embryos were later dissected to obtain hybrid plants. These plants were albinos and morphologically resembled C. pinnatifidum. Shrivelled seeds were also obtained in 2% of the crosses, which on germination gave rise to albino plants. These plants did not survive beyond 20 days. The hybrid nature of these plants was confirmed by esterase isoenzyme studies. Hybrid shoots obtained from germinating embryos were cultured on modified ML-6 medium with 2 mg benzyladenine and 0.5 mg IAA/litre, where they turned green after 3-4 weeks. Transmission electron microscopy (TEM) studies on leaf sections from green hybrid shoots showed an improvement in the chloroplast structure, with better organized grana

Item Type: Article
Author Affiliation: Cellular and Molecular Biology Division, International Crops Research Institute for the Semi-Arid Tropics, Asia Centre, Patancheru, PO 502 324, Andhra Pradesh, India
Subjects: Crop Improvement
Crop Improvement > Plant Breeding
Divisions: Chickpea
Depositing User: Mr B Krishnamurthy
Date Deposited: 25 Feb 2011 20:46
Last Modified: 25 Feb 2011 20:46
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