Development of PCR markers for wheat leaf rust resistance gene Lr47

Helguera, M. and Khan, I.A. and Dubcovsky, J. (2000) Development of PCR markers for wheat leaf rust resistance gene Lr47. TAG Theoretical and Applied Genetics, 101 (4). pp. 625-631.

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The leaf rust resistance gene Lr47 confers resistance to a wide spectrum of leaf rust strains. This gene was recently transferred from chromosome 7S of Triticum speltoides to chromosome 7A of hexaploid wheat Triticum aestivum. To facilitate the transfer of Lr47 to commercial varieties, the completely linked restriction fragment length polymorphism (RFLP) locus Xabc465 was converted into a PCR-based marker. Barley clone ABC465 is orthologous to the type-I wheat sucrose synthase gene and primers were designed for the conserved regions between the two sequences. These conserved primers were used to amplify, clone and sequence different alleles from T. speltoides and T. aestivum. This sequence information was used to identify the T. speltoides sequence, detect allele-specific mutations, and design specific primers. Cosegregation of the PCR product of these primers and the T. speltoides chromosome segment was confirmed in four backcross-populations. To complement this dominant marker, a cleavage amplified polymorphic sequence (CAPS) was developed for the 7A allele of Xabc465. This CAPS marker is useful to select homozygous Lr47 plants from F2 or backcross-F2 segregating populations, and in combination with the T- speltoides specific primers is expected to facilitate the deployment of Lr47 in new bread wheat varieties.

Item Type: Article
Author Affiliation: Department of Agronomy and Range Science, University of California, One Shields Avenue, Davis CA 95616-8515, California, USA
Subjects: Crop Improvement > Genetics/Genomics
Crop Improvement > Plant Breeding
Divisions: Other Crops
Depositing User: Sandhya Gir
Date Deposited: 07 Mar 2011 22:20
Last Modified: 07 Mar 2011 22:21
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