Ovule and embryo culture to obtain hybrids from interspecific incompatible pollinations in chickpea

Mallikarjuna, N. (1999) Ovule and embryo culture to obtain hybrids from interspecific incompatible pollinations in chickpea. Euphytica, 110 (1). pp. 1-6.

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Many of the wild species of chickpea are not accessible to the improvement of chickpea due to cross incompatibility. In these interspecific incompatible crosses, fertilization takes place but the embryo aborts a few days later. The only way to obtain hybrid plants is by the application of growth regulators to pollinated pistils to prevent initial pod abscission and to save the aborting hybrid embryos by embryo rescue techniques. Although there are a few papers on regeneration from different explants of chickpea, information on embryo rescue techniques is not available. This paper summarizes the embryo rescue techniques developed for chickpea, by the use of which hybrid plants between C. arietinum and C. pinnatifidum were produced. The study also emphasises the effects of genotype to successfully obtain hybrids. The morphology of the hybrid plants resembled the male parent in leaf structure and growth habit. The colour of the flowers produced on the hybrid plant was pale violet, resembling the male parent whose flowers were violet in colour. The flower colour of the female parent was white

Item Type: Article
Author Affiliation: Cellular and Molecular Biology Division, International Crops Research Institute for Semi-Arid Tropics, Asia Center, Patancheru, P.O. Box 502 324, Andhra Pradesh, India
Subjects: Crop Improvement > Genetics/Genomics
Crop Improvement > Plant Breeding
Divisions: Chickpea
Depositing User: Mr B Krishnamurthy
Date Deposited: 14 Mar 2011 20:33
Last Modified: 14 Mar 2011 20:33
Official URL: http://dx.doi.org/10.1023/A:1003621908663
URI: http://eprints.icrisat.ac.in/id/eprint/1617

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