Characterization of donor genome contents of backcross progenies detected by SSR markers in rice

Ying, X.Z. and FengHua, H. and RuiZhen, Z. and ZeMin, Z. and XiaoHua, D. and WenTao, L. and GuiQuan, Z. (2008) Characterization of donor genome contents of backcross progenies detected by SSR markers in rice. Euphytica, 160 (3). pp. 369-377.

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Backcrossing is a trait introgression method of renewed importance in crops. The evolution of introgressed or substituted segments through backcross generations has been analyzed theoretically using simulations. In this study, the content of donor genomes, including donor segment number (DSN), donor segment length (DSL), and donor genome size (DGS), were directly analyzed in six crosses over three successive backcrosses using a set of single sequence repeat (SSR) markers covering the entire rice genome. The results of this analysis demonstrated that the average DSN in each genome was 8.39 in BC2F1, 4.13 in BC3F1, and 2.41 in BC4F1, decreasing nearly by half with each backcrossing. The average DSL was 33.43 cM (centiMorgans) in BC2F1, 29.04 cM in BC3F1, and 25.07 cM in BC4F1, display a progressive decrease slightly greater than 10% in each additional backcross generation. Meanwhile the average DGS was 280.51 cM in BC2F1, 119.97 cM in BC3F1, and 60.53 cM in BC4F1, decreasing 57.2% from BC2F1 to BC3F1 and 50.4% from BC3F1 to BC4F1. This revealed that the reduction in DGS was approximately 50% with each backcrossing. These results provide a guide for introgression or substitution of target chromosome segments from donors into recipients in backcross programs

Item Type: Article
Author Affiliation: Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China
Subjects: Crop Improvement
Divisions: Other Crops
Depositing User: Syamala
Date Deposited: 22 Mar 2011 22:59
Last Modified: 22 Mar 2011 22:59
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