Effect of hormones, explants and genotypes in in vitro culturing of sorghum

Arulselvi, P.I. and Krishnaveni, S. (2009) Effect of hormones, explants and genotypes in in vitro culturing of sorghum. Journal Biochemical Technology , 1 (4). pp. 96-103.

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Abstract

In vitro culturing of sorghum is very much influenced by genotypes, explants, media composition and hormones. Among the genotypes tested CO 25 was found to show high frequency of callus induction and regeneration. Regeneration efficiency of embryogenic calli from CO 25 was found to be greater (88-98%) than that of calli derived from other explants (Immature inflorescence, Mature embryo and Shoot tips) of different genotypes (CO 26, TNS 586 and CO-S-28). Out of the different media tried for callus induction and regeneration of sorghum, I6 medium + kinetin 0.5 mg l-1 + 2,4-D 2 mg l-1 showed high frequency of embryogenic calli from immature embryo of CO25. Regeneration medium I6 + Kinetin (1.0) + NAA (0.5) + casein hydrolysate at levels of 250 mg l-1 and 500 mg l-1 was found to best medium composition for regeneration. An efficient protocol has been developed from suitable explant and genotype for callus induction, regeneration of sorghum and thus to produce transgenic plants for biotic and abiotic resistance

Item Type: Article
Author Affiliation: Department of Biotechnology, Periyar University, Salem, Tamil Nadu, India
Subjects: Crop Improvement
Crop Improvement > Genetics
Divisions: Sorghum
Depositing User: Mr T L Gautham
Date Deposited: 14 Apr 2011 05:46
Last Modified: 14 Apr 2011 05:46
Official URL: http://jbiochemtech.com/4two.pdf
URI: http://eprints.icrisat.ac.in/id/eprint/1752

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