Diversity in selected wild and cultivated species of pigeonpea using RFLP of mtDNA

Sivaramakrishnan, S. and Kannan, S. and Reddy, L.J. (2002) Diversity in selected wild and cultivated species of pigeonpea using RFLP of mtDNA. Euphytica, 125 (1). pp. 21-28.

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Diversity in 28 accessions representing 12 species of the genus, Cajanus arranged in 6 sections including 5 accessions of the cultivated species, C. cajan, and 4 species of the genus Rhyncosia available in the germplasm collection at ICRISAT was assessed using RFLP with maize mtDNA probes. Cluster analysis of the Southern blot hybridization data with 3 restriction enzymes - 3 probe combinations placed the genus Rhyncosia [? Rhynchosia] in a major group well separated from all the species belonging to the genus Cajanus. Within the genus Cajanus, the 4 accessions of C. platycarpus belonging to section Rhynchosoides formed a separate group in contrast to those in other sections of pigeonpea. In the section Cajanus, all the 5 accessions of C. cajan were grouped together and C. cajanifolius belonging to the same section was in a subgroup by itself closer to the main group. The four accessions of C. scarabaeoides, were together and the other species belonging to section Cantharospermum were in different subgroups. The intraspecific variation was seen even within accessions of certain pigeonpea wild species such as C. scarabaeoides, C. platycarpus, C. acutifolius, and even the cultivated species of C. cajan. This study suggests that RFLP of mtDNA can be used for the diversity analysis of pigeonpea and it gives some indications on the maternal lineage among the species. The variations in the mitochondrial DNA hybridization patterns also suggest the extensive rearrangement of the organelle genome among the Cajanus species

Item Type: Article
Author Affiliation: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, A.P. 502 324, India
Subjects: Crop Improvement > Genetics
Crop Improvement > Plant Breeding
Divisions: Pigeonpea
Depositing User: Mr B Krishnamurthy
Date Deposited: 20 Apr 2011 05:03
Last Modified: 20 Apr 2011 05:03
Official URL: http://dx.doi.org/10.1023/A:1015759318497
URI: http://eprints.icrisat.ac.in/id/eprint/1820

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