Isolation and characterization of new microsatellite markers in mango (Mangifera indica)

Honsho, Chitose and Nishiyama, Kiyomi and Eiadthong, Wichan and Yonemori, Keizo (2005) Isolation and characterization of new microsatellite markers in mango (Mangifera indica). Molecular Ecology Notes, 5 (1). pp. 152-154.

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Six microsatellite loci that were isolated from a microsatellite-enriched genomic library of mango (Mangifera indica) along with their specific primer sets were each characterized by using 36 cultivars collected mainly in Thailand. The observed and expected heterozygosities ranged from 0 to 0.83 and from 0.29 to 0.73, respectively. The number of putative alleles are two to six. Three of the six alleles have frequencies of over 75%. The high frequency may be attributed to the bias in the origin of cultivars. Among 36 mango cultivars tested, 29 cultivarsshowed a unique pattern by six primer sets, whereas seven cultivars cannot be identified because of genotype similarities. This suggests the potentials for identification of mango cultivars by microsatellite markers.

Item Type: Article
Author Affiliation: Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606–8502, Japan
Subjects: Crop Improvement
Divisions: Other Crops
Depositing User: Sandhya Gir
Date Deposited: 21 Apr 2011 08:20
Last Modified: 21 Apr 2011 08:20

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